猪繁殖与呼吸综合征病毒对Marc145细胞自噬相关基因转录的影响及分析

doi:10.11843/j.issn.0366-6964.2018.08.013

Abstract

Abstract:

Autophagy is involved in a variety of disease processes and is closely related to viral infection and proliferation. In order to explore the difference and regularity of the mRNA expression of autophagy-related genes Beclin1, ATG5, ATG12 in Marc145 cells infected with porcine reproductive and respiratory syndrome virus (PRRSV), and to correlate PRRSV proliferation with autophagy, the real-time fluorescence quantitative PCR was used. The expressions of Beclin1, ATG5 and ATG12 mRNA were detected by real-time fluorescence quantitative PCR. The proliferation of PRRSV in Marc145 cells was measured by real-time fluorescence. The results showed that the expression of autophagy-related genes in the test group were higher than those in the control group, which means that PRRSV could induce autophagy of Marc145 cells. Meanwhile, the expression of Beclin1, ATG5 and ATG12 mRNA were advanced into the high expression state; the expression principle was positively correlated with the cytopathic effect and PRRSV proliferation.

CLC Number:

S852.659.6

LI Xiao-jing, GONG Shuang-yan, MA Hai-qiang, LIU Dan, GAO Jian, PENG Xue, ZHU Ling, XU Zhi-wen. The Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Expression of Autophagy-Related Genes in Marc145 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1687-1692.

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The Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Expression of Autophagy-Related Genes in Marc145 Cells

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